described35 (Addgene inc)
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described35
Described35, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/described35/mEmerald-CD9-10+(Plasmid+%2354029)/pm39512906-211-8-11
Average 93 stars, based on 11 article reviews
Described35, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/described35/mEmerald-CD9-10+(Plasmid+%2354029)/pm39512906-211-8-11
Average 93 stars, based on 11 article reviews
described35 - by Bioz Stars,
2026-10
93/100 stars
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Clone Assay:Article Title: Large and small extracellular vesicles from Wharton’s jelly MSCs: Biophysics, function, and strategies to improve immunomodulation Article Snippet: WJ-MSC-EV-bead complexes were centrifuged and blocked with BSA 7.5% (BSA Fraction V, 7.5%, Gibco, USA) for 30 min at RT and further incubated with CD63-FITC (H5C6), CD9-PE (HI9a) and CD81 (TAPA-1)-APC (5A6) antibodies or corresponding isotype controls (BioLegend) for an additional 30 min at 4 C. Following centrifugation, pellets with lgEV and smEV beads were re-suspended in sterile-filtered PBS acquired in a BD LSR II system and analyzed with FlowJo software (Tree Star, USA). .. The CD9GFP fusion protein was cloned as previously Article Title: Large and small extracellular vesicles from Wharton's jelly MSCs: Biophysics, function, and strategies to improve immunomodulation. Article Snippet: WJ-MSC-EV-bead complexes were centrifuged and blocked with BSA 7.5% (BSA Fraction V, 7.5%, Gibco, USA) for 30 min at RT and further incubated with CD63-FITC (H5C6), CD9-PE (HI9a) and CD81 (TAPA-1)-APC (5A6) antibodies or corresponding isotype controls (BioLegend) for an additional 30 min at 4 C. Following centrifugation, pellets with lgEV and smEV beads were re-suspended in sterile-filtered PBS acquired in a BD LSR II system and analyzed with FlowJo software (Tree Star, USA). .. The CD9GFP fusion protein was cloned as previously Plasmid Preparation:Article Title: Large and small extracellular vesicles from Wharton’s jelly MSCs: Biophysics, function, and strategies to improve immunomodulation Article Snippet: WJ-MSC-EV-bead complexes were centrifuged and blocked with BSA 7.5% (BSA Fraction V, 7.5%, Gibco, USA) for 30 min at RT and further incubated with CD63-FITC (H5C6), CD9-PE (HI9a) and CD81 (TAPA-1)-APC (5A6) antibodies or corresponding isotype controls (BioLegend) for an additional 30 min at 4 C. Following centrifugation, pellets with lgEV and smEV beads were re-suspended in sterile-filtered PBS acquired in a BD LSR II system and analyzed with FlowJo software (Tree Star, USA). .. The CD9GFP fusion protein was cloned as previously Article Title: Large and small extracellular vesicles from Wharton's jelly MSCs: Biophysics, function, and strategies to improve immunomodulation. Article Snippet: WJ-MSC-EV-bead complexes were centrifuged and blocked with BSA 7.5% (BSA Fraction V, 7.5%, Gibco, USA) for 30 min at RT and further incubated with CD63-FITC (H5C6), CD9-PE (HI9a) and CD81 (TAPA-1)-APC (5A6) antibodies or corresponding isotype controls (BioLegend) for an additional 30 min at 4 C. Following centrifugation, pellets with lgEV and smEV beads were re-suspended in sterile-filtered PBS acquired in a BD LSR II system and analyzed with FlowJo software (Tree Star, USA). .. The CD9GFP fusion protein was cloned as previously |